Engineering of E. coli cells for TAG accumulation. Heterologous genes are highlighted in purple. Deleted genes are shown in red. G-3-P, glycerol-3-phosphate; Lyso-PA, lysophosphatidic acid; PA, phosphatidic acid; DAG, diacylglycerol; TAG, triacylglycerol; CDP-DAG, cytidine-diphosphate-diacylglycerol; PG-3-P, phosphatidylglycerol-3-phosphate; PG, phosphatidylglycerol; PS, phosphatidylserine; PE, phosphatidylethanolamine; WS/DGAT, wax ester synthase/acyl-CoA:diacylglycerol acyltransferase gene; RoPAP, phosphatidic acid phosphatase gene from R. opacus PD630; CrPDAT, phospholipid:diacylglycerol acyltransferase gene from C. reinhardtii; tesA, acyl-CoA thioesterase I gene; fadD, fatty acyl-CoA synthetase gene; fadE, acyl-CoA dehydrogenase gene; glpK, glycerol kinase gene; plsB, glycerol-3-phosphate acyltransferase gene; plsC, 1-acylglycerol-3-phosphate O-acyltransferase gene; dgkA, diacylglycerol kinase gene; pgpB, phosphatidylglycerophosphatase B gene; cdsA, CTP:2,3,4-saturated l-phosphatidate cytidylyltransferase gene; pgsA, phosphatidylglycerophosphate synthase gene; pgpABC, phosphatidylglycerophosphatase ABC gene; pssA, phosphatidylserine synthase gene; psd, phosphatidylserine decarboxylase gene; opgE, phosphoethanolamine transferase gene; opgB, phosphoglycerol transferase I gene; OPG, osmoregulated periplasmic glucans.