FIG 3.

Synergistic effects of CrPDAT and WS/DGAT on TAG content and fatty acid profile. (a) Thin-layer chromatography (TLC) result of neutral lipid profile. (b) Fatty acid profiles of cellular TAGs extracted from ΔΔPA and ΔΔPT. (c) Fatty acid profiles of cellular FFAs extracted from ΔΔP, ΔΔPA, and ΔΔPT. Cells were cultured in auto-induction medium with 3% glycerol at 37°C with shaking at 200 rpm for 48 h. Lipids were extracted from 20 mg of dried recombinant cells. ΔΔCDF, ΔΔBL21 harboring pCDFDuet-1; ΔΔA, ΔΔBL21 harboring pCDFDuet::atfA::RoPAP; ΔΔP, ΔΔBL21 harboring CrPDAT; ΔΔPA, ΔΔBL21 harboring CrPDAT, atfA, and RoPAP; ΔΔPT, ΔΔBL21 harboring CrPDAT, tDGAT, and RoPAP. All data are the mean ± standard deviation (shown as error bar) from triplicate samples.