TABLE 2.

Analysis of three mutants generated by rational mutagenesis of CrPDAT^a,b

Strain	Biomass (g/liter)	TAG (μg/mg CDW)	TAG (mg/liter)	FFA (mg/liter)	TFA (mg/liter)
ΔΔP	6.8 ± 0.2	0.9 ± 0.2	6.1 ± 0.8	155.9 ± 15.2	208.9 ± 9.6
Mutant phe-262-Gly	6.3 ± 0.3	1.0 ± 0.1	6.2 ± 0.5	128.6 ± 25.5c	241.4 ± 27.3
Mutant Phe-262-Ala	5.1 ± 0.8	0.8 ± 0.1	4.1 ± 0.1	93.9 ± 30.6c	131.4 ± 68.1
Mutant Gly-941-Ile	4.9 ± 0.4	0.9 ± 0.1	4.4 ± 0.3	63.1 ± 17.2c	158.9 ± 11.4

^aΔΔP, ΔΔBL21 harboring pRSF::CrPDAT; TAG, triacylglycerol; FFA, free fatty acid; TFA, total fatty acid; CDW, cell dry weight.

^bCells were cultivated in ZYP-5052 auto-induction medium with 3% glycerol at 37°C and shaking at 200 rpm for 48 h. All data are the means ± standard deviations from triplicates.

^cThe significance between ΔΔP and each mutant (Student’s t test; P < 0.05).