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. 2020 Sep 1;86(18):e00999-20. doi: 10.1128/AEM.00999-20

TABLE 3.

Evaluation of the ability of three selected PDATs to synthesize TAGa,b

Strain/ΔΔBL21 harboring: Biomass (g/liter) TAG (μg/mg CDW) TAG (mg/liter)
pRSF::CrPDAT 6.8 ± 0.1 0.9 ± 0.1 6.1 ± 0.1
pRSF::RcPDAT 5.4 ± 0.7 0.1 ± 0.0 0.5 ± 0.0
pRSF::ScPDAT 5.4 ± 0.1 1.0 ± 0.1 5.4 ± 0.1
a

CrPDAT, PDAT from C. reinhardtii; RcPDAT, PDAT from R. communis; ScPDAT, PDAT from S. cerevisiae; TAG, triacylglycerol; CDW, cell dry weight.

b

Cells were cultivated in ZYP-5052 auto-induction medium with 3% glycerol at 37°C and shaking at 200 rpm for 48 h. All data are the means ± standard deviations from triplicates.