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. Author manuscript; available in PMC: 2021 May 5.
Published in final edited form as: Cell Metab. 2020 May 5;31(5):1004–1016.e5. doi: 10.1016/j.cmet.2020.04.004

Key Resources Table.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
rat anti-insulin (pro-)/C-peptide DSHB GN-ID4
mouse anti-glucagon Santa Cruz sc-514592
guinea pig anti-insulin Dako A0564
mouse anti-Nkx6.1 DSHB F55A12
rabbit anti phospho-S6 (Ser240/244 Cell Signaling 5364
rabbit anti Mafa Cell signaling; 79737
goat anti Pdx1 R&D Systems AF2419
rabbit anti phospho-AMPKα Cell signaling 2535
Biological Samples
Cadaveric human islets Prodo labs N/A
Fetal human pancreas University of Washington N/A
Chemicals, Peptides, and Recombinant Proteins
mTeSR1 medium StemCell Technologies 85850
nutrient-free RPMI-1640 medium mybiosource MBS652918
RPMI-1640 medium with no glucose Life Technologies 11879–020
RPMI-1640 medium Life Technologies 11875–093
CMRL 1066 Supplemented Mediatech 99–603-CV
FBS (HyClone) VWR 16777
insulin Sigma 19278
S961 Novonordisk N/A
exendin-4 Sigma E7144
forskolin Santa Cruz SC3562
Torin-1 Thermo Fisher 424710
leucine Sigma N/A
diazoxide Sigma D9035
perm/fix solution BD 554714
Amino acids Sigma N/A
Critical Commercial Assays
500ml spinner flasks Corning
flow cytometry tubes BD Falcon 352235
RLT Lysis Buffer QIAGEN 79216
Human Ultrasensitive Insulin ELISA ALPCO 80-INSHUU-E1.1
TrypLE Express GIBCO 12604013
Deposited Data
mRNA sequencing of stem cell-derived beta cells before and after transplantation into mice GEO NCBI GSE147346
mRNA sequencing of stem cell-derived beta cells cultured in different AA concentrations GEO NCBI GSE147347
single-cell mRNA sequencing of human embryonic pancreas GEO NCBI GSE147349
Experimental Models: Cell Lines
HUES8 human embryonic stem cells NIH #0021
Experimental Models: Organisms/Strains
C57BL/6 Jackson Laboratory N/A
SCID-Beige mice Jackson Laboratory N/A
Prkaa1tm1.1Sjm/J Jackson Laboratory 014141
Prkaa2tm1.1Sjm/J Jackson Laboratory 014142
Tsc1tm1Djk/J Jackson Laboratory 005680
B6.Cg-Tg(Ins1-cre/ERT)1Lphi/J Jackson Laboratory 024709
Oligonucleotides
Custom Taqman probes for TSC1, TSC2, LKB1, PRKAA1, PRKAA2, SESN1, SESN2, UBC and GAPDH Applied Biosystems
Cre forward: GAACCTGATGGACATGTTCAGG
Cre reverse: AGTGCGTTCGAACGCTAGAGCCTGT
Prkaa1 forward: CCC ACC ATC ACT CCA TCT CT
Prkaa1 reverse: AGC CTG CTT GGC ACA CTT AT
Prkaa2 forward: GCA GGC GAA TTT CTG AGT TC
Prkaa2 reverse: TCC CCT TGA ACA AGC ATA CC
TSC1 forward: GTC ACG ACC GTA GGA GAA GC
TSC1 reverse: GAA TCA ACC CCA CAG AGC AT
Recombinant DNA
Software and Algorithms
Biorender https://app.biorender.com/
Prism Graphpad https://www.graphpad.com/scientific-software/prism/
ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/
FlowJo FlowJo https://www.flowjo.com
Other