Figure 2.

S. japonicum egg-derived EVs inhibit HSCs activation via high expression of Sja-miR-71a. (a). Expression levels of Sja-miR-71a in mice livers in different stages of S. japonicum infection were analysed by qRT-PCR (n = 4 per group). (b). Sja-miR-71a expression in serum EVs of patients with schistosomiasis japonicum was analysed by qRT-PCR (n = 9–10 per group). (c, d). HSCs (LX2) were treated with PBS, TGF-β1 (10 ng/mL), and TGF-β1 (10 ng/mL) + Sja-miR-71a (50 nM) for 72 h. α-SMA and Collagen I expression were analysed by qRT-PCR and western blotting. Results are shown as mean ± SD. (a): Kruskal–Wallis test; (b): Unpaired two-sample t-test; (c and d): One-way ANOVA.