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. 2020 Jul 9;9(1):1785738. doi: 10.1080/20013078.2020.1785738

Figure 6.

Figure 6.

Sja-miR-71a suppresses liver fibrosis via Sema4D/TGF-β1 and Sema4D/IL-13 axes. (a). Immunohistochemical analysis of TGF-β1, phosphorylated SMAD2/3 (p-SMAD2/3) and SMAD4 in mice livers, and the sum of the IOD was analysed by Image-Pro Plus 6.0 (n = 5–9 per group). (b). Expression of IL-13Rα1, phosphorylated JAK1 (p-JAK1) and phosphorylated SATA6 (p-SATA6) in mice livers were analysed by immunohistochemistry, and the sum of the IOD was analysed by Image-Pro Plus 6.0 (n = 5–9 per group). (c, d). HSCs (LX2) were treated with PBS, TGF-β1 (10 ng/mL) and TGF-β1 (10 ng/mL) +Sja-miR-71a (50 nM) for 72 h; p-SMAD 2/3 and SMAD4 were analysed by immunofluorescence analysis and western blotting. (e, f). HSCs (LX2) were treated with PBS, TGF-β1 (10 ng/mL) and TGF-β1 (10 ng/mL)+Sja-miR-71a (50 nM) for 72 h; IL-13Rα1, p-JAK1 and p-SATA6 were analysed by immunofluorescence analysis and western blotting. Results are shown as mean ± SD. One-way ANOVA test was used for statistical analysis.