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. 2020 Aug 10;9(1):1800971. doi: 10.1080/20013078.2020.1800971

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Characterization of hESCs and hESC-sEVs.

(a) Representative bright-field image and OCT4, NANOG, SSEA-4, TRA-1-60, TRA-1-81 immunostaining micrographs of hESCs. Nucleus was stained with DAPI. Scale bar, 50 μm. (b) Representative TEM images of hESC-sEVs. Scale bar, 100 nm. (c) Average particle size distribution and concentration of hESC-sEVs measured by qNano. (d) Representative western blot analysis showing the presence of exosomal markers including CD9, CD63, and TSG101, but negative for GM130 in hESC-sEVs. (e,f) Evaluation of hESC-sEVs yield in term of particle concentration. (g,h) Evaluation of hESC-sEVs yield in term of protein concentration. Each experiment was repeated three to five times independently. Data are represented as mean±s.e.m.