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. 2020 Jun 18;9(1):1781427. doi: 10.1080/20013078.2020.1781427

Figure 1.

Figure 1.

Morphology of EVs released from rat aortic EC and VSMC. Rat aortic EC (a) and VSMC (b) were cultured under serum-free conditions. EVs released into the media were isolated by ultracentrifugation, fixed to Formvar carbon-coated copper grids, stained and observed under transmission electron microscopy. Images were taken at either 200,000 (a) or 80,000 (b) magnification. (c) ECs or (d) VSMCs were cultured with serum-free DMEM containing GW4869 (GW: 20 μmol/L) or the vehicle (vehi: DMSO 0.1%) for 48h. EVs were isolated from the culture media and NanoSight (NS300 NTA) analysis was used to determine the ratio of released EV particles. The bars in the graphs show the mean±SEM from three independent experiments. (e and f) Immunoblot analysis of equal loading of EC and VSMC whole cell lysates (WC) and EV preparations. Tsg 101; tumour susceptibility gene-101, VDAC; voltage-dependent anion-selective channel-1. Representative data are shown from three independent experiments. c* indicates p < 0.05.