Figure 4.

EC EVs stimulate protein synthesis in VSMCs. VSMCs were incubated with EC-derived EVs (5 x10⁸ particles) for indicated time points. Samples were pulsed with 5 µmol/L puromycin for 30 min at the end of the experiment and processed for Western blot analysis using an anti-body against puromycin. Cycloheximide (cyc: 10 µg/mL for 30 min treatment) was used to inhibit protein synthesis as control (n = 3 for 1.5 h, n = 4 for remainder). * indicates p < 0.05.