Figure 1.
Conceptual frameworks for hypotheses of environmental factors inducing immune tolerance by HLA-G during pregnancy and the verification procedure using Ex-vivo Culture System. (a) In vivo Environmental factors for promoting HLA-G gene transcription, various HLA-G isoforms expression, and secretion consistently. (b) Hypotheses that encourage EVT-specific HLA-G transcription, sustained HLA-G1/2 expression and soluble HLA-G5/6 secretion at the maternal-foetal interface during pregnancy and in vitro culture conditions for demonstrating them. (c) Flowchart for verification of hypotheses by inducing immune-tolerized Stem Cells (itSCs) and immune-tolerized EVs (itSC-EVs) secreted from them using maternal-foetal interface-like ex-vivo culture system (HA-MatrixDecidua).