Figure 7.

T-cell Proliferation Assay and T_reg-cell differentiation Assay of MSC^Control-EVs and MSC^Matrix+T/V-EVs (itSC-EVs). CFSE pre-labelled PBMCs were stimulated with 5 μg/ml of PHA and co-cultured with MSC^Control-EVs and MSC^Matrix+T/V-EVs for 5 days, respectively. Each EVs were obtained from the same number of MSCs with PBMCs. (a) MSC^Matrix+T/V-EVs are much more effective to inhibit the proliferation of CD3⁺ T-cell than MSC^Control-EVs on PHA-stimulated PBMCs. (b) MSC^Matrix+T/V-EVs have a remarkable effect on the induction of CD3⁺/CD25⁺ T_reg-cell differentiation from total CD3⁺ T-cell compared with MSC^Control-EVs. Error bars represent SD. **, P < 0.01; the Student’s t-test.