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. 2020 Aug 19;9(1):1806444. doi: 10.1080/20013078.2020.1806444

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Characterization of M-EV and exosomes isolated from DC2.4 cells and human serum, respectively. (a, b) Nanoparticle tracking analysis (NTA) of M-EV and exosomes (EXO) isolated from the cell culture and human serum, respectively. (c) Transmission electron micrographs of M-EV and EXO. Scale bar: 50 nm. (d) Western blot of EV markers CD81 (~26 kDa), CD47 (~50 kDa), and CD63 (~53 kDa) on M-EV and EXO. (e) Confocal fluorescence imaging of DiO/DiI-loaded targeted vesicles incubated with A549 cells (green: DiO, red: DiD; scale bar: 10 μm). (f) FRET efficiency of hybrid and no-hybrid targeted vesicles.