Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 26;9(1):1798606. doi: 10.1080/20013078.2020.1798606

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Subcellular distributions of HLA II and CD63 relative to phagocytosed E. coli.

Representative flattened maximum intensity 3D SIM images from one experiment out of three independent experiments. (a) The subcellular distributions of HLA II (grey) in an immature moDC (left panel) and in an activated moDC after 16-hour incubation in the presence of Cy3B-labelled E. coli (cyan) (middle panel). Note the redistribution of HLA II from endosomal/lysosomal compartments to the plasma membrane in response to activation. The indicated segment is enlarged in the right panel, together with YZ and XZ sections of the same segment. Note the punctate HLA II staining surrounding E. coli-containing phagosomes. For 3D rotation see supplementary movie 1. (b) The subcellular distributions of the exosomal marker CD63 (grey) in an immature moDC (left panel) and in an activated moDC (middle panel) upon uptake of E. coli (cyan). The indicated segment is enlarged in the right panel, together with YZ and XZ sections of the same segment. Note the recruitment of CD63 towards the phagosome containing area of the moDC, and the punctate staining pattern surrounding the many E. coli-containing phagosomes. For 3D rotation see supplementary movie 2.