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. 2020 Jul 26;9(1):1798606. doi: 10.1080/20013078.2020.1798606

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — EV markers on expulsed E. coli.

MoDC were cultured for 16 hour in presence of Cy3B-labelled E. coli. Extracellular E. coli (cyan) that were captured together with moDC were often decorated with HLA II (grey) and CD63 (grey) puncta (left panels), indicating associated EV. Merged pictures show three and two representative examples for HLA II and CD63 respectively. Single channel pictures are displayed in half size prints for comparison. The edge of one MoDC is indicated with c. Examples of control E. coli that had not been incubated with moDC were imaged with identical settings, and the absence of HLA II and CD63 labelling on these EV lacking bacteria demonstrates specificity of the labelling procedure (right panels, indicated with no MoDC).