Figure 6.

LEV treatment downregulates inflammation-induced expression of the M1 macrophage cell-surface marker, HLA-DRα. Human THP1 monocytes at day 1 post plating were pre-incubated with 10 nM PMA. PMA was maintained during the treatment period (A) or washed out after 48 h (b). On days 3 and 5, the cells were treated with LEVs (10 μg/ml) or 20 ng/ml of IFN-γ and 10 pg/ml of LPS for 48 h. On day 7, the cells were harvested and HLA-DRα mRNA expression was analysed by RT-qPCR. G6PD was used for normalization. Data are expressed as the mean fold change ± SEM of triplicate measurements and statistical significance was analysed by one-way ANOVA.

*p < 0.05, ***p < 0.001; ns, non-significant.