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. 2020 Jul 13;9(1):1790159. doi: 10.1080/20013078.2020.1790159

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — IL-8 is present on the surface of sEVs. (A) Conditioned medium derived from A431-Dsg2/GFP cells was used to isolate sEVs by sequential ultracentrifugation and iodixanol separation. Fractions were collected from the top and immunoblotted for CD63. (B) Fraction 4, enriched in CD63, was digested with trypsin (0.2%) and then subjected to Western blotting analysis for IL-8 and GAPDH.