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. 2020 Jun 16;9(1):1778883. doi: 10.1080/20013078.2020.1778883

Figure 4.

Figure 4.

hUC-MSCs-sEVs activated the PTEN/AKT signalling pathway by delivering miR-23a-3p. (a) Normalized miRNA expression levels measured by miRNA microarray. (b) Normalized chondrogenesis-related miRNA expression levels and gene expression level of miR-23a-3p. (c) The potential target sequences of miR-23a-3p were predicted by bioinformatic analysis. (d) 293 T cells were transfected with luciferase reporter plasmids WT or MUT 3ʹ-UTR of PTEN and miR-23a-3p, luciferase activity was detected by Dual-Luciferase Reporter Assay System. Data were presented as mean ± SD of three number of replicates. **P < 0.01. (e) Chondrocytes were transfected miR-23a-3p for 24 h, then the expression of miR-23a-3p in chondrocytes was measured by qPCR. Data were presented as mean ± SD of three number of replicates. t-test was applied to each group in order to compare mean beta values. ***P < 0.001. (f) The protein levels of AKT, P-AKT, PTEN in chondrocytes were quantified using western blotting.