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. 2020 Sep 2;5(3):231–245. doi: 10.1089/can.2019.0047

Table 1.

Summary of Mouse Studies

Strain Sex Vendor Response Schedule CB1R agonist Dose Tested phenotype Post-inj. time Sig. Ref.
ddYO M Kuyodo, Saga, Japan Initial Sensitivity Acute THC 10 mg/kg (i.p.) Immobility (bar test) 60 min ** 34
ddYO M Kiwa Experimental Animal Laboratories, Wakayama, Japan Initial Sensitivity Acute THC 6 mg/kg (i.p.) Motor Depression (activity chamber) 60 min * 28
Hypothermia 180 min *  
10 mg/kg (i.p.) Motor Depression (activity chamber) 60 min **  
Hypothermia 60 min **
Hypothermia 180 min *
ICRO M Harlan Laboratories, Indianapolis, IN Initial Sensitivity Acute Cannabis† Dose–response (10, 50, 100, 200, and 300 mg, inhalation) Motor Depression (activity chamber) 5 min n.s. 31
Antinociception (twl) 20 min ED50=60 mg*  
Immobility (ring test) 40 min ED50=103 mg*  
Hypothermia 60 min n.s.
Initial sensitivity Acute THC Dose–response (0, 0.5, 1, 2, 4, and 8 mg/kg, i.v.) Motor Depression (activity chamber) 5 min ED50=NA‡*
Antinociception (twl) 20 min ED50=2.8 mg/kg*
Immobility (ring test) 40 min ED50=1.9 mg/kg*
Hypothermia 60 min ED50=3.4 mg/kg*
Swiss WebsterO M University of Arkansas for Medical Sciences Colony Initial Sensitivity Acute THC Dose–response (0,30, and 100 mg/30 L, inhalation) Hypothermia 0 min 30 and 100 mg/30 L* 33
Antinociception (twl) 0 min 100 mg/30 L*  
Catalepsy (bar test) 0 min n.s.  
Motor Depression (activity chamber) 0 min 100 mg/30 L*
THC Dose–response (0, 10, 30, and 100 mg/kg, i.p.) Hypothermia 0 min *
Antinociception (twl) 0 min *
Catalepsy (bar test) 0 min 30 and 100 mg/kg*
Motor Depression (activity chamber) 0 min *
JWH-018 (10, 30, and 100 mg/30 L, inhalation) Hypothermia 0 min 10, 30, and 100 mg/30 L*
Antinociception (twl) 0 min 30 and 100 mg/30 L*
Catalepsy (bar test) 0 min n.s.
Motor Depression (activity chamber) 0 min 10, 30, and 100 mg/30 L*
JWH-018 Dose–response (0, 1, 3, and 10 mg/kg, i.p.) Hypothermia 0 min *
Antinociception (twl) 0 min *
Catalepsy (bar test) 0 min 3 and 10 mg/kg*
Motor Depression (activity chamber) 0 min *
JWH-073 (10,30, and 100 mg/30 L, inhalation) Hypothermia 0 min 30 and 100 mg/30 L*
Antinociception (twl) 0 min 100 mg/30 L*
Catalepsy (bar test) 0 min n.s.
Motor Depression (activity chamber) 0 min 30 and 100 mg/30 L*
JWH-073 Dose–response (0, 3, 10, 30 mg/kg, i.p.) Hypothermia 0 min *
Antinociception (twl) 0 min *
Catalepsy (bar test) 0 min 30 mg/kg*
Motor Depression (activity chamber) 0 min *
C57BL/6I F Jackson Laboratories, Bar Harbor, ME Initial Sensitivity Acute THC 10 mg/kg (i.p.) Motor Depression (activity chamber) 35 min Y*; A*** 30
M 5 mg/kg (i.p.) A*  
10 mg/kg (i.p.) A**  
ICRO M Charles River Laboratories, Wilmington, MA Initial Sensitivity Acute THC 50 mg/kg (i.p.) Catalepsy (ring test) 60 min * 35
Hypothermia 30 min *  
Motor Depression (activity chamber) 0 min *  
Antinociception (twl) 20 min *
C57BL/6I M Charles River Laboratories, Wilmington, MA Initial Sensitivity Acute THC 50 mg/kg (i.p.) Catalepsy (ring test) 60 min *
Hypothermia 30 min n.s.
Motor Depression (activity chamber) 0 min *
Antinociception (twl) 20 min n.s.
DBA/2I M Charles River Laboratories, Wilmington, MA Initial Sensitivity Acute THC 50 mg/kg (i.p.) Catalepsy (ring test) 60 min *
Hypothermia 30 min *
Motor Depression (activity chamber) 0 min *
Antinociception (twl) 20 min *
ICRO M Harlan Laboratories, Indianapolis, IN Tolerance Repeated THC 0.5 day: 10 mg/kg (x1 daily s.c) + dose–response 24 h later (i.v.) Antinociception 20 min n.s.; ED50=1.10 mg/kg 27
Motor Depression (activity chamber) 5 min n.s.; ED50=0.61 mg/kg  
1.5 day: 10 mg/kg (x2 daily s.c.) for 1 day +10 mg/kg (x1 daily s.c) + dose–response 24 h later (i.v.) Antinociception 20 min ED50=5.75 mg/kg*  
Motor Depression (activity chamber) 5 min ED50=4.16 mg/kg*
3.5 day: 10 mg/kg (x2 daily s.c.) for 3 days +10 mg/kg (x1 daily s.c) + dose–response 24 h later (i.v.) Antinociception 20 min ED50=8.76 mg/kg*
Motor Depression (activity chamber) 5 min ED50=17 mg/kg*
6.5 day: 10 mg/kg (x2 daily s.c.) for 6 days +10 mg/kg (x1 daily s.c) + dose–response 24 h later (i.v.) Antinociception 20 min ED50=9.14 mg/kg*
Motor Depression (activity chamber) 5 min ED50=8.09 mg/kg*
C57BL/6JI M Jackson Laboratories, Bar Harbor, ME Tolerance Repeated THC 2 days (10 mg/kg i.p. x1 every 72 h) Motor Depression (open field) 0 min J*; A** 29
DBA/2JI M Jackson Laboratories, Bar Harbor, ME Tolerance Repeated THC 2 days (10 mg/kg i.p. x1 every 72 h) Motor Depression (open field) 0 min A*  
C57BL/6JArcI M Animal Resource Centre, Australia Initial Sensitivity Acute THC 0.3, 1, 3 mg/kg (i.p.) Catalepsy (bar test) 20 min n.s. 32
Hyperthermia 30 min n.s.  
Antinociception (twl) 45 min n.s.  
Motor Depression (open field) 30 min n.s.
10 mg/kg (i.p.) Catalepsy (bar test) 20 min *
Hypothermia 30 min *
Antinociception (twl) 45 min n.s.
Motor Depression (open field) 30 min *
Tolerance Repeated THC 3, 5, 7, 12 or 14 consecutive treatment days at 0.3, 1, 3, or 10 mg/kg (i.p.) per day Catalepsy (bar test) 20 min n.s.
Hypothermia 30 min n.s.
14 consecutive treatment days at 0.3, 1, or 3 mg/kg (i.p.) per day Antinociception (twl) 45 min n.s.
14 consecutive treatment days at 10 mg/kg (i.p.) per day Antinociception (twl) 45 min ***
15 and 21 consecutive treatment days at 0.3, 1, or 3 mg/kg (i.p.) per day Motor Depression (open field) 30 min Day 21; 1 mg/kg* and 3 mg/kg**
15 and 21 consecutive treatment days at 10 mg/kg (i.p.) per day Motor Depression (open field) 30 min Day 15** and Day 21***

Post-inj. Time=Time point trait was measured relative to CB1R agonist exposure. Sig.=Significance level; p<0.05*, p<0.01**, p<0.001***. Ref.=Reference cited. twl=tail withdrawal latency in response to a thermal stimulus. J=juvenile (32 days old) and A=adult (73 days old). n.s.=not significant.

†

Cannabis contained 3.46% THC, 0.18% cannabinol, 0.17% cannabidiol, 0.14% cannabigerol, and 0.05% tetrahydrocannabivarin.

I

Inbred strain.

O

outbred strain.

‡

THC significantly inhibited locomotor activity; EC50 was not calculated because an effect >50% was not observed at the highest dose.