Effect of cannabis extract treatment on body weight gain, food intake, and liver tissue and adipose tissue weight in mice fed HFCD. Male C57BL/6J mice aged 7–8 weeks were fed HFCD. Mice were intubated three times a week with CN1 or CN2 or CN6 cannabis extracts in concentrations of 5 mg/kg for 6 weeks. (A) Body weight over experiment duration. (B) Body weight gain over experiment duration. (C) Average food intake per mouse per day over the experiment duration. (D) Liver weight of mice at sacrifice. (E) Adipose tissue weight of mice at sacrifice. All values are expressed as mean±SEM (n=8). Columns marked with different letters (a, b) are significantly different (p<0.05) according to the Tukey–Kramer post hoc test. *Different from ND. #Different from HFCD (p<0.05) according to Student's t-test. HFCD, high-fat/cholesterol diet; ND, normal diet; SEM, standard error of the mean.