Table 2.

Genotyping conditions for vitamin D receptor single nucleotide polymorphism (VDR SNPs)

VDR SNP	Primers	Polymerase chain reaction (PCR) conditions	Fragment sizes (bp)	Restriction enzyme*
ApaI (rs7975232)	5′-GGATCCTAAATGCACGGAGA-3′ 5′-ACGTCTGCAGTGTGTTGGAC-3′	1. 1 × : 95 °C (5 min) 35 × : 2. 95 °C (60 s) 3. 55 °C (60 s) 4. 72 °C (60 s) 5. 1 × : 72 °C (10 min)	PCR product: 265 T allele: 265 G allele: 146 + 119	ApaI
BsmI (rs1544410)	5′-CCTCACTGCCCTTAGCTCTG-3′ 5′-TGCCTCCAAAATCAATCAGG-3′		PCR product: 247 A allele: 247 G allele: 144 + 103	BsmI
Cdx2 (rs11568820)	5′-GGATCCCAAAAGGAAAGGAA-3′ 5′-TGTTCCAGATGGTAAAAATAGAATGA-3′		PCR product: 396 A allele: 316 + 80 G allele: 265 + 80 + 51	HpyCH4III
TaqI (rs731236)	5′-CAGAGCATGGACAGGGAGCAA-3′ 5′-CACTTCGAGCACAAGGGGCGTTAGC-3′		PCR product: 501 T allele: 495 + 6 C allele: 294 + 201 + 6	TaqI
FokI (rs2228570)	5′-AGCTGGCCCTGGCACTGACTCTGCTCT -3′ 5′-ATGGAAACACCTTGCTTCTTCTCCCTC -3′	1. 1 × : 94 °C (5 min) 35 × : 2. 94 °C (35 s) 3. 61 °C (35 s) 4. 72 °C (1 min) 5. 1 × : 72 °C (7 min)	PCR product: 267 C allele: 267 T allele: 195 + 72	FokI

*New England Biolabs (Ipswich, MA, USA).