Table 1.

Direct antiglobulin and drug dependent RBC antibody testing.

Test	Case 1 Result	Case 2 Result
ABO Rh	O Rh Positive	O Rh Negative
DAT Polyspecific	Positive (2 +)	Negative
DAT Anti-IgG	Negative	Negative
DAT Anti-C3d	Positive (1 +)	Negative
DAT Anti-C3b, C3d	Positive (4 +)	Negative
DAT Saline Control	Negative	Negative
New Antibodies in Serum	Autoanti-I reactive at IS, RT, 37 °C	Autoanti-I reactive at 18 °C, 4 °C
Ceftriaxone dependent RBC antibody	Negative	Negative
Hydroxychloroquine dependent RBC antibody	Negative	Negative

Initial testing performed by University of North Carolina McClendon Laboratories in Chapel Hill, NC. Confirmatory testing and drug-dependent antibody testing performed by Versiti in Milwaukee, WI.

DAT: Direct antiglobulin test. RBC: Red blood cell. IS: Immediate spin. RT: Room temperature.