Fig 6. Rho1 promotes tissue bending and regulation of cell height.

(A-B”) MS1096>RyR^RANi was used as a control (n = 5) for the comparisons. (A-A”) Representation of immunohistochemistry data using standard deviation z plane projections along with cross-sectional views along a line parallel to the AP axis. (C-D”) Loss of function of Rho1 in the wing imaginal disc was introduced using the Rho1^RNAi line (BL# 27727). (C-C”) Data corresponding to A-A”. (E) Rho1^RNAi (n = 8) in the wing imaginal discs leads to an increased columnar cell height as compared to control discs (n = 5). (F) Inhibition of Rho1 in the wing imaginal discs leads to flattening of the discs quantified through Menger curvature. A sample size of 5 and 8 was used for the control and Rho1^RNAi discs. (p-values of a student’s t-test included in plots) (G-I”) MS1096-Gal4 is expressed preferentially in the dorsal compartment of the wing imaginal disc. (G-H”‘) Representation of immunohistochemistry data using standard deviation z plane projections along with cross-sectional views along a line parallel to the D-V boundary. (H–I”‘) Cross-sectional views of the wing disc along lines parallel to the D-V boundary and located above and below the DV boundary as indicated by the yellow +δ and—δ lines in G.