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. 2020 Sep 9;15(9):e0238637. doi: 10.1371/journal.pone.0238637

Fig 3. Growth is further stunted by combined exposure to heat stress and “T+S”.

Fig 3

Cross-sectional area of day 5 C. elegans raised on “T+S” or control plates was measured 8 hours after exposure to either paraquat (a known oxidative stressor), 35˚C heat stress, or a water control. C. elegans were imaged and then measured using Image J/FIJI software. A total of 36–64 worms were analyzed for each treatment group or control. Data were analyzed using a 1-way ANOVA. “T+S” significantly reduced body size (p<0.0001), as did heat stress (p<0.0001). Heat stress significantly increased the effect of “T+S” (n = 190, p<0.001). In contrast, paraquat did not affect body size (n = 122, p = 0.93) and the combination of both “T+S” and paraquat had no additional effect (p = 0.1911).