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. 2020 Aug 27;16(8):e1008327. doi: 10.1371/journal.ppat.1008327

Fig 7. IL-12 signaling is partially required for TGD057-specifc CD8 T cell IFNγ responses.

Fig 7

(A) Il12b-/- (IL-12p40) BMDMs were infected with clade D ME49, or (B) clade B MAS and TGD057-specific CD8 T cell IFNγ responses were measured as described in Fig 1A. Each dot represents the result of an individual experiment and the average of 4 experiments + SD is shown; statistical analysis was performed with an unpaired two-tailed t-test; ** p ≤ 0.01. (C) Various Pru (clade D) gene deletion strains, Δgra15, Δgra24, and Δgra15 Δgra24, or (D) a Pru strain transgenically expressing clade A ROP16 (Pru +ROP16A) were assayed for TGD057-specific CD8 T cell IFNγ responses. The IFNγ response, as analyzed by ELISA, is normalized to that induced by the wildtype Pru strain. Each dot represents the result of an individual experiment and the average of 3–6 experiments + SD is shown. Statistical analysis was performed using one-way ANOVA with Bonferroni’s correction for (C), and an unpaired two-tailed t-test for (D); * p ≤ 0.05.