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. 2020 Sep 9;15(9):e0237981. doi: 10.1371/journal.pone.0237981

Fig 4. Analysis of glycine and proliferation in vitro as a function of SHMT2 and sodium benzoate.

Fig 4

HeLa-Ss cells were grown in normal media under SHMT2 Tet induction (Up) or IPTG knock-down (Down) treatment for 72 h. Intracellular metabolites were extracted and analyzed by selected reaction monitoring mass spectrometry, SRM, n = 5 to 8 biological replicates. (A) In HeLa-Ss cells relative levels of intracellular glycine levels were measured as a function of endogenous SHMT2 expression (control; C) or elevated (+) or decreased (–) expression of SHTM2 (described above), and without (–) or with (+) glycine supplementation in growth medium. (B) HeLa-Ss cell viability as a function of sodium benzoate concentration as indicated in uninduced cells (control, cont) or following induction of ectopic SHMT2 (Up) or knockdown of SHMT2 expression (Down).