Table 1. Proteins with significantly high biotinylation in cells expressing SHMT2-BirA.
| Gene | Protein |
*Amount Log2 [SHMT2/Control) |
SHMT2 Association Rank |
|---|---|---|---|
| SHMT2 | Serine hydroxymethyltransferase, mitochondrial | 14.24 | |
| ACOT2 | Acyl-coenzyme A thioesterase 2, mitochondrial | 12.42 | 1 |
| ACOT1 | Acyl-coenzyme A thioesterase 1 | 10.73 | 2 |
| HSPE1 | 10 kDa heat shock protein, mitochondrial | 10.71 | 3 |
| NDUFV3 | NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial | 10.26 | 4 |
| LETM1 | LETM1 and EF-hand domain-containing protein 1, mitochondrial | 9.90 | 5 |
| NDUFAF2 | Mimitin, mitochondrial | 9.45 | 6 |
| NNT | NAD(P) transhydrogenase, mitochondrial | 9.29 | 7 |
| AFG3L2 | AFG3-like protein 2 | 8.95 | 8 |
| MDH2 | Malate dehydrogenase, mitochondrial;Malate dehydrogenase | 8.27 | 9 |
| TACO1 | Translational activator of cytochrome c oxidase 1 | 8.26 | 10 |
| IARS2 | Isoleucine—tRNA ligase, mitochondrial | 8.05 | 11 |
| HINT2 | Histidine triad nucleotide-binding protein 2, mitochondrial | 8.00 | 12 |
| PYCR1 | Pyrroline-5-carboxylate reductase 1, mitochondrial | 8.00 | 13 |
| NFS1 | Cysteine desulfurase, mitochondrial | 7.61 | 14 |
| GTPBP10 | GTP-binding protein 10 | 7.40 | 15 |
| HSD17B10 | 3-hydroxyacyl-CoA dehydrogenase type-2 | 7.39 | 16 |
| SUCLA2 | Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial | 7.36 | 17 |
| ECH1 | Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial | 7.28 | 18 |
| ALDH1L2 | Mitochondrial 10-formyltetrahydrofolate dehydrogenase | 7.25 | 19 |
| GLS | Glutaminase kidney isoform, mitochondrial | 7.22 | 20 |
| GATM | Glycine amidinotransferase, mitochondrial | 7.21 | 21 |
| SDHA | Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial | 7.14 | 22 |
| TRMT10C | Mitochondrial ribonuclease P protein 1 | 6.94 | 23 |
| ACAD9 | Acyl-CoA dehydrogenase family member 9, mitochondrial | 6.92 | 24 |
| NDUFS3 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial | 6.83 | 25 |
| GLUD1 | Glutamate dehydrogenase, mitochondrial | 6.75 | 26 |
| ETFB | Electron transfer flavoprotein subunit beta | 6.72 | 27 |
| TIMM44 | Mitochondrial import inner membrane translocase subunit TIM44 | 6.63 | 28 |
| ISCU | Iron-sulfur cluster assembly enzyme ISCU, mitochondrial | 6.62 | 29 |
| UQCRB | Cytochrome b-c1 complex subunit 7 | 6.61 | 30 |
| PPA2 | Inorganic pyrophosphatase 2, mitochondrial | 6.52 | 31 |
| NDUFAF4 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4 | 6.47 | 32 |
| HIBCH | 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial | 6.44 | 33 |
| CLPX | ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial | 6.37 | 34 |
| NDUFA12 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 | 6.34 | 35 |
| NDUFV2 | NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial | 6.27 | 36 |
| GFM1 | Elongation factor G, mitochondrial | 6.25 | 37 |
| AK3 | GTP:AMP phosphotransferase AK3, mitochondrial | 6.20 | 38 |
| FASTKD2 | FAST kinase domain-containing protein 2 | 6.13 | 39 |
| OXSM | 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial | 6.08 | 40 |
| ATP5B | ATP synthase subunit beta, mitochondrial | 5.93 | 41 |
| MRPS28 | 28S ribosomal protein S28, mitochondrial | 5.87 | 42 |
| RTN4IP1 | Reticulon-4-interacting protein 1, mitochondrial | 5.81 | 43 |
| MMAB | Cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial | 5.71 | 44 |
| LYRM7 | Complex III assembly factor LYRM7 | 5.62 | 45 |
| MGME1 | Mitochondrial genome maintenance exonuclease 1 | 5.55 | 46 |
| ATPAF1 | ATP synthase mitochondrial F1 complex assembly factor 1 | 5.27 | 47 |
| DBT | Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial | 5.20 | 48 |
*Proteins with >32 fold increased biotinylation in SHMT2-BirA cells compared with control (Cont)-BirA and CHCHD2-BirA cells (n = 3, p<0.05)