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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Biomaterials. 2020 Jun 15;256:120182. doi: 10.1016/j.biomaterials.2020.120182

Figure 3. Alginate Encapsulated Cells Effectively Activate Antigen-Specific CD8+ T cells via the Indirect Antigen Recognition Pathway.

Figure 3.

(A) Schematics of the 48hr coculture experiment co-incubating mOVA stimulator cells, either unencapsulated or encapsulated form, with the unsorted OTI splenic immune responder cells. OVA-specific OTI CD8+ T cell activation was quantified by the % of proliferating and granzyme B + CD8+ T effectors via flow cytometry analysis (see Figure S2). Representative FCM data of OVA-specific CD8+ T cell activation within the unsorted OT1 responder pool in response to the equivalent amount of unencapsulated (B) or alginate encapsulated (C) mOVA cells. (D) Summary of the frequency of proliferating granzyme B+ CD8+ effector T cells, gated from the whole OTI splenocyte population, in response to designated stimuli (x-axis). Bars indicate the average with individual data points (N=4; n=6-11) shown with standard deviation. Statistical difference was determined as ****p < 0.0001; n.s. = not significant via Tukey’s test when compared with the unstimulated control group.