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. Author manuscript; available in PMC: 2021 May 21.
Published in final edited form as: Integr Biol (Camb). 2020 May 21;12(5):109–121. doi: 10.1093/intbio/zyaa008

Figure 2.

Figure 2.

Model calibration and validation. The model was fit to experimental data for (A) pSFK, (B) pZAP70, (C) pSLP76, (D) pLAT, (E) pPLCγ, (F) pVav, (G) pErk and (H) pAkt. The model predictions were validated against separate data for (I) pErk, (J) pAkt, (K) pPLCγ and (L) pVav under co-stimulation of 2B4 and NKG2D. Blue: CD16 pathway; purple: 2B4 pathway; orange: NKG2D pathway; Green and Brown: 2B4 and NKG2D co-stimulation from separate experiments. Note that the green and brown lines represent independent western blot experiments that only differ in the time-points of data collection. Solid lines: mean model predictions from 1000 parameter estimates. Shaded area: standard deviation of mean model predictions. Squares, circles and triangles: experimental data.