Fig. 1.

In HSPCs of d715 Csf3r mice, RUNX1 mutations decrease CFU-G and CFU-GM formation but increase the replating capacity. a Schematic of RUNX1 protein showing the location and amino acid changes of the mutations, which are indicated by black triangles. The functionally important Runt homology DNA-binding domain (RHD) is shown in blue, and the transactivation domain (TAD) is shown in red. b Schematic of CFU experiments performed using transduced bone marrow lin⁻ cells of C57BL/6-d715 Csf3r mice. c Representative WB images of lin⁻ cells of C57BL/6-d715 Csf3r mice transduced with corresponding lentiviral constructs. GAPDH was used as loading control. d CFU assay and e replating CFU assay of transduced C57BL/6-d715 Csf3r lin⁻ cells. Data represent means ± SD from triplicates of two independent experiments; *p < 0.05, **p < 0.01,*** p < 0.001