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. 2020 Sep 9;11:4520. doi: 10.1038/s41467-020-18298-8

Fig. 7. High-collagen expression correlates with decreased CD8+ T cells, increased LAIR1 and TIM-3 exhaustion markers in human lung cancer datasets.

Fig. 7

a Representative images of human lung cancer tissue sections (n = 451 for TMA and 8–10 whole tissue sections) stained by IHC for collagen I, collagen III, CD8, and TIM3. Scale bars, 200 µm. Inset scale bars, 50 µm. b Cluster plot analysis of Spearman’s rank correlation between total percent collagen I & III area versus CD8 or TIM-3 IHC signal count in human lung cancer tissue microarrays (n = 451 tissue samples). c Cluster plot analysis of Spearman’s rank correlation between percent collagen I or collagen III area versus CD8 or TIM3 IHC signal count in human lung cancer whole tissue sections (n = 8–10 tissue samples). d Heatmap showing the association of mRNA expression in the TCGA LUSC dataset between LAIR1 and collagen genes that are statistically significant (P < 0.05) by Spearman’s rank correlation. e Heatmap showing the association of mRNA expression in TCGA LUSC dataset between HAVCR2 (TIM-3) and collagen genes that are statistically significant (P < 0.05) by Spearman’s rank correlation. f Representative cluster plot analysis of Spearman’s rank correlation between COL3A1 versus LAIR1 or HAVCR2 (TIM3) mRNA expression in the TCGA LUSC dataset. g Cluster plot analysis of Spearman’s rank correlation between LAIR1 versus HAVCR2 (TIM3) mRNA expression in the TCGA LUSC dataset.