Skip to main content
. 2020 Sep 9;11(9):731. doi: 10.1038/s41419-020-02952-6

Fig. 3. miR-210-3p directly targets GPD1L and CYGB in TNBC.

Fig. 3

a Predictive target genes of miR-210-3p from TargetScan, miRDB, and miRBase. b Western blotting analysis of GPD1L, CYGB and FGFRL1 protein expression in MDA-MB-231 and Hs578T cells after transfection of miR-210-3p; β-actin was loaded as an internal control. c Predicted miR-210-3p target sequences in 3′-UTR of GPD1L; a mutant 3’UTR with substitutions in the complementary site for the seed region of miR-210-3p was used as a control. d Luciferase reporter activity in MDA-MB-231 and Hs578T cells co-transfection of the luciferase reporter vector (GPD1L-WT or GPD1L-Mut) and miR-210-3p mimics; luciferase activity was measured 48 h after transfection. e Predicted miR-210-3p target sequences in 3′-UTR of CYGB; a mutant 3′-UTR with substitutions in the complementary site for the seed region of miR-210-3p was used as a control. f Luciferase reporter activity in MDA-MB-231 and Hs578T cells co-transfection of the luciferase reporter vector (CYGB-WT or CYGB-Mut) and miR-210-3p mimics; luciferase activity was measured 48 h after transfection. g Correlation analysis between miR-210-3p and CYGB/GPD1L expression in 22 TNBC samples. *p < 0.05; **p < 0.01.