Figure 1.

Strongly Expressed Integral Membrane Substrates Cause a Growth Defect in dfm1Δ Cells

(A) Galactose-induced Hmg2-GFP overexpression causes a growth defect in dfm1Δ cells. WT, dfm1Δ, and hrd1Δ cells either containing empty vector or GAL-driven Hmg2-GFP were compared for growth by dilution assay. Each strain was spotted 5-fold dilutions on glucose or galactose-containing plates to drive Hmg2-GFP overexpression, and plates were incubated at 30°C.

(B–F) WT, dfm1Δ, and hrd1Δ cells were spotted in 5-fold dilutions on glucose or galactose-containing plates to drive overexpression of 6xmyc-Hmg2-GFP, Sec61-2-GFP, Pdr5∗-HA, SUS-GFP, and CPY∗-HA.

(G and H) Same as (A) except non-passaged dfm1Δ and dfm1Δ hrd1Δ non-passaged cells (P0) or cells passaged to suppression (P11) were assessed for growth defect in the dilution assay.

(I) Same as (A) except dilution assay was performed using galactose-induced overexpression of WT Sec61-GFP or mutant Sec61-2-GFP.

(J) Same as (A) except dilution assay was performed on WT, dfm1Δ, ire1Δ, dfm1Δ ire1Δ cells using GAL-driven Hmg2-GFP.