Fig. 2. Genes with interconnected functions show correlated phenotypic signatures.

a–f Genes within a shared functional pathway show relationships in their Tn-seq fitness signatures. a, c Heat maps (using blue-white-red scale at bottom) show normalized Tn-seq fitness in z-scored units for mutants in each gene (rows) grown in distinct conditions (columns). Characterized/annotated genes were first placed into shared functional pathways (Methods). We next identified additional genes (arrowheads), many of which were uncharacterized/unannotated, that correlate with each pathway by using hierarchical clustering with the entire set of A. baumannii phenotypic signatures. Parentheses denote domains identified from NCBI conserved domain database (CDD)⁴⁶. In c, dashed black lines separate genes with opposing regulatory effects. b, d Heat maps (using yellow-white-blue scale at bottom) show Pearson correlation coefficient (r) matrices measuring relatedness of the corresponding Tn-seq fitness signatures in (a). Positive and negative r indicate correlation and anticorrelation, respectively. e, f Phenotypic signatures correlating with pAB3-encoded ACX60_RS18565. Tn-seq fitness (e) and correlations (f) are shown as above. N/A, no gene name or predicted protein domain. Source data are provided as a Source Data file.