Figure 6.

Glycolytic stress in cultured GC cells is relieved by siMAO-A. (A). Western blot analysis of HK II and PDH in AGS cells in the indicated groups. (B). The immunoblotting of HK II and PDH in MGC803 cells after MAO-A knockdown. (C–D). Extracellular acidification rates of cultured AGS and MGC803 cells measured by Seahorse XFe24 to assess glycolysis following 10 mM glucose, 1 µM oligomycin and 100 mM 2-DG treatments. n = 5. *P < 0.05, **P < 0.01 compared with NC. All of the values are presented as the means ± SEM.

Abbreviations: AGS and MGC803, human gastric cancer cell lines; 2-DG, 2-deoxy-D-glucose; GC, gastric cancer; HK II, hexokinase II; NC, non-targeted control; PDH, pyruvate dehydrogenase; ROS, reactive oxygen species; siMAO-A, small interfering RNA of MAO-A.