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. 2020 Sep 4;13:8825–8835. doi: 10.2147/OTT.S257056

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© 2020 Wang et al.

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TRPM8 expression in BCa tissues. (A) Immunofluorescence staining for TRPM8 (green) in normal bladder tissues, paracancerous tissues and BCa tissues. OCT-4 (red) was used as a marker of BCa cells, and nuclei were stained by DAPI (blue). The scale bar represents 250 μm. (B) qRT-PCR analysis of the expression of TRPM8 mRNA in BCa (n = 17) and matched paracancerous tissues (n = 17). (C) Increased expression of TRPM8 in BCa tissues from the TCGA database was analyzed. (D) qRT-PCR validated the efficacy of knockdown at mRNA level by TRPM8 target-specific siRNA (siTRPM8) in BCa T24 cells. (E) Western blot analysis validated the efficacy of knockdown at the protein level by the TRPM8 target-specific siRNA (siTRPM8) in T24 BCa cells. *p<0.05.