FIGURE 4.

Fermentation result of the engineered EP-bifido strains. (A) Growth curve of first batch selection. (B) Growth curve of second batch selection. (C) Growth curve of third batch selection. (D) PHB content and glucose consumption of these three batches selection. The blue columns represent PHB content and orange red broken line represents glucose consumption. (E) PHB content and yield of selected recombinants. The experiments were performed in triplicate and error bars indicate s.d. For mutant fermentation selection, strains were cultivated with M9 at 37°C, 150 rpm. (F) Growth curve of selected recombinants.