TABLE 1.
Bacterial strains and plasmids used in this study.
| Strain and plasmids | Relevant properties | Sources |
| Strains | ||
| JW1087-2 | JW25113 derivative, ΔptsG:kan | Baba et al. (2006) |
| DH-EP | DH5α derivative, ΔeddΔpfkA | Wang et al. (2019) |
| DH-EPP | DH5α derivative, ΔeddΔpfkAΔptsG | This study |
| L-6 | DH-EPP derivative, ΔeddΔpfkAΔptsG galP RBS:TGAAAGGGAAA | This study |
| M-6 | L-6 derivative, ΔeddΔpfkAΔptsG:trc-rbs-glfzm | This study |
| Plasmids | ||
| pCAB | pBluescriptII SK, phbC and phbAB gene from Ralstonia eutropha | Wang et al. (2019) |
| pCDFtrc | Cloning vector, SpeR | Wang et al. (2019) |
| pFF | pCDFtrc, fxpk gene from B. adolescentis and fbp gene from E. coli | Wang et al. (2019) |
| pKD3 | Template plasmid with CmR gene and FLP recognition target | Datsenko and Wanner (2000) |
| pTKRED | PBAD promoter containing plasmid, SpeR | Kuhlman and Cox (2010) |
| pCP20 | Helper plasmid expressing FLP recombinase, ts-rep, AmpR, CmR | Datsenko and Wanner (2000) |
| pAH69 | Helper plasmid expressing HK022 integrase, AmpR | Haldimann and Wanner (2001) |