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. 2020 Aug 13;10(22):10231–10244. doi: 10.7150/thno.48410

Figure 4.

Figure 4

IGF-1 mimetic peptide inhibits the foam cell formation in vitro. (A) Peritoneal macrophages collected from apoE-/- mice were stained with Oil Red O to assess formation of foam cells (>10 lipid droplets per cell, >10 fields per sample), n = 5. (B) Expression of ABCA1 and ABCG1 in total cellular extract was determined by western blot after being treated with or without 10 nM of diluted gel with H1, H2, H3, H4 and IGF-1 protein overnight. Quantification of the relative levels of ABCA1 or ABCG1 versus GAPDH in each sample was determined by ImageJ, as a fold of control, n = 5. (C) HASMCs were treated with 10 nM of diluted gel with H1, H2, H3, H4 and IGF-1 protein overnight. αSMA expression was analyzed by immunofluorescent staining with quantitation of αSMA-positive cells, n=5. (D) HASMCs were treated with 10 nM of diluted gel with H1, H2, H3, H4 and IGF-1 protein in the absence or presence of ox-LDL (50 µg·mL-1) overnight. Expression of CD68 and SM22α was determined by qRT-PCR, n = 5. Data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001 significantly different from control group or as indicated.