Figure 2.

PML-RARα-positive APL cells induced lipid production in hepatocytes. (A) Approaches to define the effects of murine APL cells or C1498 AML cells on lipid production and LDLR expression in hepatocytes. (B) Total triglyceride (TG) and cholesterol levels in culture supernatants from mouse hepatocytes were determined by enzymatic assay. (C) The PCSK9 levels in the culture supernatants of mouse hepatocytes cocultured with the indicated cells were detected by ELISA. (D) Confocal assay of LDLR expression in mouse hepatocytes cocultured with C1498 AML or APL cells. (E) Approaches to define murine serum TG/HDL/LDL levels in myeloid cell-specific Pml-Rarα knockin (Pml-Rarα^KI) mice (3-4 months old). (F-H) Serum TG, HDL, and LDL levels in WT mice and Pml-Rarα^KI mice at the age of 3-4 months.