Figure 4.

Reduced PPARγ activity in APL cells causes lipid metabolism disorder in APL patients. (A) Correlation between PML-RARα and PPARγ expression (protein level) in human primary APL cells (n = 16). Each data point represents the value from an individual patient. Statistical significance was measured by Pearson's correlation test. (B) PML-RARα decreased the transcriptional activity of PPARγ. HEK 293T cells were transiently transfected with the indicated plasmids. After 24 h of transfection, luciferase activities were measured. (C) Approaches to define the effects of C1498 AML cells with or without PPARγ depletion on the liver and blood lipid levels of C57 BL/6 recipient mice. (D-G) Liver and serum TG and TC levels in mice transplanted with CTRL shRNA C1498 cells or PPARγ shRNA C1498 cells at the indicated times after inoculation. For panel D-G, n = 4 mice per group.