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D@PNE effectively induced ICD of 4T1 cells and elicited DC maturation in vitro. (A) Extracellular HMGB-1 release and (B) ATP secretion of 4T1 cells with different treatments for 2 h. (C) CRT exposure of 4T1 cells with different treatment for 4 h. (D) Quantification and (E) representative flow cytometry plots of the mature DCs (CD11c+CD80+CD86+) after various treatments in vitro. The DOX concentration was 1µg/mL; *p < 0.05, **p < 0.01, ***p < 0.001.
