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. 2020 Aug 8;10(22):10016–10030. doi: 10.7150/thno.47081

Figure 4.

Figure 4

GSK-J4 downregulates stemness-associated gene signatures in CRC. (A) Volcano plot showing differentially expressed genes (DEGs) from RNA-seq analysis of HCT116 cells treated with or without 15 µm GSK-J4 for 48 h. Genes with fold change ≥ 2 and false discovery rate (FDR) ≤ 0.01 were considered as significant DEGs. (B) Heatmap showing expression level of DEGs described in (A) across indicated samples. (C) Results of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of DEGs described in (A). (D-F) Results of GSEA using specified gene sets and transcriptomic data described in (A). FDR ≤ 0.25 were considered as statistically significant. NES, normalized enrichment score.