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. 2020 Aug 27;8:849. doi: 10.3389/fcell.2020.00849

FIGURE 2.

FIGURE 2

Inflammatory conditions abolish the effect of TRPV4 inhibition on transendothelial electrical resistance (TEER) in mouse brain microvascular endothelial cells (MbMECs). (A) TEER of MbMECs treated with 5 nM of GSK2193874 in comparison to vehicle-treated MbMECs, both were exposed to 50 U/ml TNFα and INFγ. One representative experiment out of 14 is shown. (B–D) Quantification of TEER of MbMECs treated with 5 nM of GSK2193874 (n = 14) in comparison to vehicle-treated MbMECs (n = 14) at 6, 12, and 24 h, both were exposed to 50 U/ml TNFα and INFγ. TEER was normalized to the time-point of GSK treatment (0 h). (E) Relative Trpv4 mRNA expression assessed by qPCR in MbMECs exposed to 50 U/ml TNFα and INFγ (n = 4) in comparison to homeostatic conditions (n = 8). Data are presented as box plots. Statistical analysis was performed by two-tailed Student’s t-test (A) and (B–D) or two-tailed Mann Whitney test (E). *P < 0.05.