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. 2020 Aug 27;11:1866. doi: 10.3389/fimmu.2020.01866

Figure 4.

Figure 4

Serine deprivation promotes glucose uptake in M1 macrophages. (A) Glucose uptake in TGPMs with completed medium (M1) or serine deprived medium (M1-S) (n = 6). Data of two independent experiments were analyzed with unpaired t-test and represented as means ± SEM. (B) The secretion of IL-1β and TNF-α from TGPMs culturing in completed medium, serine deprived medium, serine deprived medium supplemented with extra glucose (5/10mM) for 15 h (n = 3). Data are representative of two independent experiments. (C) The secretion of IL-1β from TGPMs culturing in completed medium, serine deprived medium, serine deprivation medium with extracellular supplementation (Left) or intracellular transfection (Right) with sodium pyruvate (10 mM) alone or combined with glucose (5 mM) for 15 h (n = 3–4). Data are representative of two independent experiments. Thioglycolate-elicited peritoneal macrophages (TGPMs) were stimulated with LPS (1 μg/ml) plus IFN-γ (20 ng/ml) for 15 h except as indicated. Data were analyzed with unpaired t-test (A) or with one-way ANOVA (B,C) and represented as mean ± SD except indicated. *P < 0.05, **P < 0.01.