Skip to main content
. 2020 Aug 27;11:1866. doi: 10.3389/fimmu.2020.01866

Figure 6.

Figure 6

Serine-free diet reduces the mouse inflammatory responses in vivo. (A) The level of IL-1β and TNF-α in the serum of mice with control diet and serine-free diet (n = 8). (B) The secretion of IL-1β from TGPMs with control diet and serine-free diet in M1 condition (n = 5). (C) The secretion of IL-1β from TGPMs with control diet and serine-free diet in M1-S condition (n = 5). (D) Immunoblotting to detect the protein abundance of mTOR, p-mTOR, p-4EBP1, p-S6K1, NLRP3, and Caspase1 in TGPMs (n = 5). (E) The levels of IL-1β and TNF-α in the serum at 15 h post LPS challenge (10 mg/kg body weight) (n = 8). (F) The levels of IL-1β and TNF-α in the lung, liver, jejunum and colon at 15 h post LPS challenge (10 mg/kg body weight) (n = 8). (G) H&E staining to analyze the lung inflammation in mice with control diet (n = 9) or serine-free diet (n = 7) at 15 h post LPS challenge (10 mg/kg body weight). M1-S: thioglycolate-elicited peritoneal macrophages (TGPMs) were stimulated with LPS (1 μg/ml) plus IFN-γ (20 ng/ml) in serine deficiency medium; M1: TGPMs were stimulated with LPS (1 μg/ml) plus IFN-γ (20 ng/ml) in completed medium. Macrophages were stimulated with LPS plus IFN-γ for 15 h except indicated. Data were analyzed with unpaired t-test and represented as means ± SD except indicated. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.