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. 2020 Aug 27;11:955. doi: 10.3389/fgene.2020.00955

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Copyright © 2020 Cheng, Fu, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The bulk m6A RNA methylation and METTL3 expression are increased in DLBCL. (A) The bulk m6A RNA methylation in 18 DLBCL tissues and 18 inflammatory lymph glands. ^∗P < 0.05. (B) The bulk m6A RNA methylation in DLBCL cell lines (SU-DHL4, OCILy10, Farage, U2932, HBL1) and Human B lymphocyte (GM12878). ^∗∗P < 0.01. (C) mRNA expression of METTL3 in 18 DLBCL tissues and 18 inflammatory lymph glands was analyzed by qRT-PCR. ^∗∗P < 0.01. (D) METTL3 expression in TCGA database between DLBCL tissues and normal counterparts. ^∗P < 0.05. (E) qRT-PCR was used to analyze mRNA expression of METTL3 in DLBCL cell lines (SU-DHL4, OCILy10, Farage, U2932, HBL1) and Human B lymphocyte (GM12878). ^∗∗P < 0.01. (F) METTL3 expression in 4 DLBCL tissues and 4 inflammatory lymph glands was analyzed by western blotting. ^∗∗P < 0.01. (G) mRNA expression of METTL14 in 18 DLBCL tissues and 18 inflammatory lymph glands was analyzed by qRT-PCR. ^∗∗P < 0.01. (H) qRT-PCR was used to analyze mRNA expression of METTL14 in DLBCL cell lines (SU-DHL4, OCILy10, Farage, U2932, HBL1) and Human B lymphocyte (GM12878). ^∗∗P < 0.01. (I) METTL14 expression in TCGA database between DLBCL tissues and normal counterparts. ^∗P < 0.05. (J) mRNA expression of WTAP in 18 DLBCL tissues and 18 inflammatory lymph glands was analyzed by qRT-PCR. ^∗P < 0.05. (K) qRT-PCR was used to analyze mRNA expression of WTAP in DLBCL cell lines (SU-DHL4, OCILy10, Farage, U2932, HBL1) and Human B lymphocyte (GM12878). ^∗P < 0.05. (L) WATP expression in TCGA database between DLBCL tissues and normal counterparts. ^∗P < 0.05.