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. 2020 Sep 10;18:130. doi: 10.1186/s12951-020-00688-1

Table 2.

Key measures for the sandwich LFA

Sandwich assay 0–20 nM Green channel
QD-525-anti-CRP
Red channel
QD-605-anti-IL-6
Image processing software ImageJ MultiFlow app ImageJ MultiFlow app
LOB Negative 0.33 Negative 0.46
LOD 2.28 1.27 1.38 2.28
LOQ 11.12 5.52 9.49 7.33
R2 of linear fit 0.89 0.95 0.83 0.97