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. 2020 Aug;9(4):1138–1148. doi: 10.21037/tlcr-19-701

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2020 Translational Lung Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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LINC00511 suppresses expression of miR-150-5p. (A,B) Subcellular fractionation and FISH assays determined the localization of LINC00511 in LUSC cells. (C) Six miRNAs that might bind with LINC00511 was screened out from starBase website. (D) Pearson’s correlation analysis examined the correlation of the six candidate miRNAs expression and LINC00511 expression in LUSC tumors one by one. (E) The expression of miR-150-5p in LUSC cells was measured by qRT-PCR. (F) qRT-PCR revealed miR-150-5p level after LINC00511 being silenced in H226 and SK-MES-1 cells. (G) The relationship between LINC00511 and miR-150-5p was disclosed by RIP assay. (H) The underlying binding sites between LINC00511 and miR-150-5p were probed by starBase website. (I) The confirmation between LINC00511 and miR-150-5p was testified by luciferase reporter assay. **P<0.01.