Table 2.
Amino acid substitutions in the internal proteins of A(H1N1)pdm09 viruses
|
Internal proteins |
Amino acid substitutions |
Bulgarian strains carrying these substitutions |
No. of strains detected in other Balkan countries carrying these substitutions |
|---|---|---|---|
|
PB2 |
T81I; G225S; V667I |
A/Bulgaria/013/2019 |
Albania (4), Macedonia (7) |
|
R299K; T398I; P453T |
All 5 Bulgarian strains |
Albania, Macedonia, Serbia (All) |
|
|
PB1 |
V200I; K386R |
A/Bulgaria/013/2019 |
Albania (4), Macedonia (7) |
|
PA |
C8S; R356K; D394N; E688G |
A/Bulgaria/1381/2018; A/Bulgaria/1527/2018 |
|
|
NP |
V319I |
A/Bulgaria1381/2018; A/Bulgaria/1527/2018 |
Albania (1), Macedonia (3) |
|
V431I |
A/Bulgaria/013/2019 |
Albania (4), Macedonia (7) |
|
|
M1 |
A166T |
A/Bulgaria1381/2018; A/Bulgaria/1527/2018 |
|
|
M2 |
E14G |
A/Bulgaria/1463/2019 |
Serbia (3) |
|
S23N |
A/Bulgaria/013/2019 |
Albania (4), Macedonia (7) |
|
|
NS1 |
M65T |
All 5 Bulgarian strains |
Albania, Macedonia, Serbia (All) |
|
T80A |
A/Bulgaria/013/2019 |
Albania (4), Macedonia (7) |
|
|
I111M |
A/Bulgaria/1463/2019 |
Serbia (3) |
|
|
A155T |
A/Bulgaria/013/2019 |
Albania (3), Macedonia (7) |
Five Bulgarian viruses (A/Bulgaria/1381/2018, A/Bulgaria/1521/2018, A/Bulgaria/1527/2018, A/Bulgaria/013/2019 and A/Bulgaria/021/2019) underwent whole-genome sequencing. Encoded internal protein sequences were compared with those of A/Michigan/45/2015 (EPI_ISL_200780) and other viruses from Albania (EPI_ISL_380103 - EPI_ISL_380107), Macedonia (EPI_ISL_362268 - EPI_ISL362274) and Serbia (EPI_ISL_393798 - EPI_ISL_393802).